Back بأر متساوي التكهرب Arabic Isoelectroenfocament Catalan Izoelektrická fokusace Czech Isoelektrische Fokussierung German Isoelectroenfoque Spanish متمرکزسازی ایزوالکتریک Persian Focalisation isoélectrique French Isoelectroenfoque Galician Isoelettrofocalizzazione Italian 等電点電気泳動 Japanese
Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI).[1][2] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the pH of its surroundings.[3]
- ^ Bjellqvist, Bengt; Ek, Kristina; Righetti, Pier Giorgio; Gianazza, Elisabetta; Görg, Angelika; Westermeier, Reiner; Postel, Wilhelm (1982). "Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications". Journal of Biochemical and Biophysical Methods. 6 (4): 317–339. doi:10.1016/0165-022X(82)90013-6. ISSN 0165-022X. PMID 7142660.
- ^ Pier Giorgio Righetti (1 April 2000). Isoelectric Focusing: Theory, Methodology and Application. Elsevier. ISBN 978-0-08-085880-7.
- ^ David Edward Garfin (1990). Isoelectric focusing. Methods in Enzymology. Vol. 182. pp. 459–77. doi:10.1016/0076-6879(90)82037-3. ISBN 9780121820831. PMID 2314254.