The Lost Colony or Roanoke Colony was the first English colony in the New World, which was abandoned and the colonists never found.
Lost Colony may also refer to:
- Lost Colony (play), a play based on the history of Roanoke Colony
- Artemis Fowl: The Lost Colony (2006), a fantasy novel by Eoin Colfer
- Deadlands: Lost Colony, a tabletop role-playing game published by Pinnacle Entertainment Group
- Wraiths of Roanoke or Lost Colony, a 2007 supernatural film about the English colony
- "The Lost Colony", a 1993 episode of the Lovejoy
- Lost Colony Entertainment, an American-based production company