The Second Division Knock-Out is a football competition that takes place in Gozo, Malta. It is organized by Gozo Football Association.
The cup holders are Victoria Hotspurs which won the cup after beating 4-0 Żebbuġ Rovers in the final held on April 6, 2024. This competition is played on a knock-out basis between the clubs of the Second Division. The first Second Division Knock-Out took place in the season 1958–1959.