A Twinkie is a snack cake with a cream filling.
Twinkie(s) may also refer to:
- Twinkie (slur), a pejorative term
- Twinkie Clark (b. 1954), a musician
- Twinkies, a nickname for the Minnesota Twins
- Twinkie, a character from the Fast & Furious franchise most notably from The Fast and the Furious: Tokyo Drift