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Transfobia adalah serangkaian perlakuan dan perasaan antagonistik melawan kaum transgender atau transseksual, atau melawan transeksualitas. Transfobia dapat membentuk perasaan tidak nyaman, takut, dan emosional pada orang-orang yang tidak nyaman terhadap keberadaan penyimpangan gender di masyarakat tersebut.[1][2] Sifat tersebut sering kali dipancarkan bersama dengan pandangan homofobik dan sering kali menjadi sebuah aspek dari homofobia.[3][4] Transfobia adalah sebuah jenis preyudis dan diskriminasi yang mirip dengan rasisme dan seksisme,[5] dan transgender kulit berwarna sering kali menjadi subyek dari seluruh tiga bentuk diskriminasi pada suatu waktu.[6]
Because of the complicated interplay among gender identity, gender roles, and sexual identity, transgender people are often assumed to be lesbian or gay (See Overview: Sexism, Heterosexism, and Transgender Oppression). ... Because transgender identity challenges a binary conception of sexuality and gender, educators must clarify their own understanding of these concepts. ... Facilitators must be able to help participants understand the connections among sexism, heterosexism, and transgender oppression and the ways in which gender roles are maintained, in part, through homophobia.
Transgender people subjected to violence, in a range of cultural contexts, frequently report that transphobic violence is expressed in homophobic terms. The tendency to translate violence against a trans person to homophobia reflects the role of gender in attribution of homosexuality as well as the fact that hostility connected to homosexuality is often associated with the perpetrators' prejudices about particular gender practices and their visibility.